Angle PLC - EACR 2025: Data Demonstrating DNA Dual Analysis

Rhea-AI Summary

ANGLE plc presented new data at EACR 2025 showcasing their DNA dual analysis workflow for comprehensive liquid biopsy profiling in lung cancer patients. The study, conducted using ANGLE's Parsortix system and Illumina's NextSeq2000 platform, analyzed both circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) using a 79-gene lung cancer panel. The research demonstrated high sensitivity in detecting clinically relevant mutations in contrived samples. In the analysis of 27 lung cancer patients, the study revealed significant differences between CTC-DNA and ctDNA, with 53% of mutations found only in CTCs, 36% only in ctDNA, and 11% in both. The findings highlighted ANGLE's unique capability to identify druggable targets through CTC-DNA analysis, including mutations in genes like CHEK2, ESR1, NTRK1, and RET, which correspond to various targeted therapies from major pharmaceutical companies.

Positive

• Study demonstrated high sensitivity in detecting clinically relevant mutations using Parsortix system
• Successfully identified druggable mutations corresponding to existing targeted therapies from major pharmaceutical companies
• Revealed 53% of mutations were found exclusively in CTCs, showing unique value proposition
• Collaboration with Illumina enables broad NGS-based molecular profiling integration

Negative

• None.

ANGLE presents new data at EACR 2025 DEMONSTRATING DNA DUAL ANALYSIS FOR COMPREHENSIVE LIQUID BIOPSY PROFILING

Poster highlights ANGLE's combined CTC-DNA and ctDNA analysis revealing tumour heterogeneity in lung cancer patients

GUILDFORD, SURREY / ACCESS Newswire / June 17, 2025 / ANGLE plc (AIM:AGL)(OTCQX:ANPCY), a world-leading liquid biopsy company with innovative circulating tumour cell (CTC) solutions for use in research, drug development and clinical oncology, is pleased to announce the presentation of a poster at the European Association for Cancer Research (EACR) Congress, taking place in Lisbon, Portugal from 16-19 June 2025.

The poster entitled 'Comparative molecular analysis of CTCs and cfDNA Detection using Parsortix^® system' is being presented during the Biomarkers in Tissue and Blood session on Tuesday 17 June 2025.

This poster builds on work first presented in a February 2025 webinar hosted by Illumina as part of a co-marketing initiative. It showcases an end-to-end Illumina-based DNA dual analysis workflow for the analysis of CTCs harvested using ANGLE's Parsortix system, alongside circulating tumour DNA (ctDNA), using a 79-gene lung cancer panel on the Illumina NextSeq2000 platform. Illumina is the world's largest provider of NGS systems and assays, with an installed base of over 25,000 sequencing systems across more than 9,500 customers in 155 countries. The study provides a practical example of how Illumina customers could integrate DNA dual analysis of CTCs and ctDNA into existing sequencing pipelines for deeper insights into tumour heterogeneity.

The first phase of the study established analytical sensitivity and specificity using contrived samples, where cancer cells were added at defined levels (0, 2, 5, 10, and 20 cells) to blood from healthy volunteers. The samples were processed with the Parsortix system, and DNA from isolated cells was analysed for seven clinically relevant mutations. Druggable mutations such as BRAF, EGFR, and TP53 were consistently detected confirming high sensitivity of the workflow.

The second phase of the study analysed blood from 27 lung cancer patients. DNA was extracted from both CTCs and plasma in each sample, then sequenced using a 79-gene lung cancer panel. The results showed substantial differences between the two analytes with 53% of mutations found in CTCs alone, 36% in ctDNA alone and 11% found in both analytes. These findings evidence that CTC-DNA and ctDNA provide distinct and complementary information, with many druggable targets found in CTC-DNA including CHEK2 (olaparib from AstraZeneca and Merck and talazoparib from Pfizer), ESR1 (elacestrant from Menarini), NTRK1 (larotrectinib from Bayer and entrectinib from Roche) and RET (selpercatinib from Eli Lilly and pralsetinib from Blueprint Medicines).

This study demonstrates ANGLE's unique ability to deliver DNA dual analysis from a single blood sample, revealing clinically relevant mutations missed by ctDNA alone. By enabling a more complete view of tumour genetics, molecular analysis of CTC-DNA and ctDNA using the Parsortix system offers biopharma partners a valuable tool for identifying resistance, stratifying patients, and improving trial outcomes.

The poster will be available on ANGLE's website from 20:00 on Tuesday 17 June: https://angleplc.com/resources/posters/

ANGLE Chief Scientific Officer, Karen Miller, commented:
"This study demonstrates the unique value of ANGLE's DNA dual analysis approach, which combines sequencing of CTC-DNA and ctDNA from the same blood sample to uncover critical differences in tumour biology. Building on our collaboration with Illumina, this workflow enables broad NGS-based molecular profiling and delivers insights that can help pharma customers identify drug targets, treatment resistance, stratify patients and minimise invasive tissue biopsy requirements for study participants."

For further information:

ANGLE plc	+44 (0) 1483 343434
Andrew Newland, Chief Executive Ian Griffiths, Finance Director
Berenberg (NOMAD and Broker) Toby Flaux, Ciaran Walsh, Milo Bonser	+44 (0) 20 3207 7800
FTI Consulting Simon Conway, Ciara Martin Matthew Ventimiglia (US)	+44 (0) 203 727 1000 +1 (212) 850 5624

For Research Use Only. Not for use in diagnostic procedures.

For Frequently Used Terms, please see the Company's website on https://angleplc.com/investor-relations/glossary/

Notes for editors

About ANGLE plc

ANGLE is a world-leading liquid biopsy company with innovative circulating tumour cell (CTC) solutions for use in research, drug development and clinical oncology using a simple blood sample. ANGLE's FDA cleared and patent protected CTC harvesting technology known as the Parsortix^® PC1 System enables complete downstream analysis of the sample including whole cell imaging and proteomic analysis and full genomic and transcriptomic molecular analysis.

ANGLE's commercial businesses are focusing on clinical services and diagnostic products. The clinical services business is offered through ANGLE's GCLP-compliant laboratories. Services include custom made assay development and clinical trial testing for pharma. Products include the Parsortix system, associated consumables and assays.

Over 100 peer-reviewed publications have demonstrated the performance of the Parsortix system. For more information, visit www.angleplc.com

Any reference to regulatory authorisations such as FDA clearance, CE marking or UK MHRA registration shall be read in conjunction with the full intended use of the product:

The Parsortix^® PC1 system is an in vitro diagnostic device intended to enrich circulating tumor cells (CTCs) from peripheral blood collected in K₂EDTA tubes from patients diagnosed with metastatic breast cancer. The system employs a microfluidic chamber (a Parsortix cell separation cassette) to capture cells of a certain size and deformability from the population of cells present in blood. The cells retained in the cassette are harvested by the Parsortix PC1 system for use in subsequent downstream assays. The end user is responsible for the validation of any downstream assay. The standalone device, as indicated, does not identify, enumerate or characterize CTCs and cannot be used to make any diagnostic/prognostic claims for CTCs, including monitoring indications or as an aid in any disease management and/or treatment decisions.

This information is provided by Reach, the non-regulatory press release distribution service of RNS, part of the London Stock Exchange. Terms and conditions relating to the use and distribution of this information may apply. For further information, please contact rns@lseg.com or visit www.rns.com.

SOURCE: ANGLE plc

View the original press release on ACCESS Newswire

FAQ

What did ANGLE plc present at EACR 2025 regarding their DNA dual analysis?

ANGLE presented data demonstrating their combined CTC-DNA and ctDNA analysis workflow using the Parsortix system, revealing tumor heterogeneity in lung cancer patients through a 79-gene panel on Illumina's NextSeq2000 platform.

How effective was ANPCY's Parsortix system in detecting mutations in lung cancer patients?

The study of 27 lung cancer patients showed the Parsortix system was highly effective, with 53% of mutations found exclusively in CTCs, 36% in ctDNA alone, and 11% in both analytes.

What druggable targets did ANGLE's CTC-DNA analysis identify in the study?

The analysis identified druggable targets including CHEK2, ESR1, NTRK1, and RET mutations, corresponding to treatments from companies like AstraZeneca, Pfizer, Menarini, Bayer, and Eli Lilly.

How did ANGLE validate the sensitivity of their DNA dual analysis workflow?

ANGLE validated the workflow using contrived samples with defined levels of cancer cells (0, 2, 5, 10, and 20 cells) in healthy volunteer blood, consistently detecting druggable mutations like BRAF, EGFR, and TP53.

What is the significance of ANGLE's collaboration with Illumina for DNA dual analysis?

The collaboration enables Illumina customers to integrate DNA dual analysis of CTCs and ctDNA into existing sequencing pipelines, providing deeper insights into tumor heterogeneity and improving cancer research outcomes.