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Bone Biologics Expands Trial Sites

Bone Biologics doubles Australian trial sites for NB1 and highlights funding runway and shelf life as it advances toward a potential pivotal U.S. study.

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Bone Biologics (BBLG) expanded its first-in-human pilot clinical trial of NB1 to six hospital sites in Australia, doubling the number of participating centers.

The larger site network is expected to accelerate patient screening and surgical scheduling and supports the company’s expectation to complete enrollment by year-end 2026. NB1, the lead rhNELL-1-based bone graft candidate, combines recombinant human NELL-1 protein with demineralized bone matrix for spinal fusion procedures.

The multicenter, prospective, randomized trial will evaluate safety and preliminary effectiveness in up to 30 adults with degenerative disc disease undergoing TLIF. Primary endpoints are fusion success at 12 and 24 months and change from baseline in the Oswestry Disability Index. The FDA’s Division of Orthopedic Devices has reviewed the study design in a pre-submission, and the pilot is intended to support a future pivotal U.S. study. The company cites cash runway through Q2 2027 and a 32‑month validated shelf life for rhNELL-1.

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Positive

  • Six Australian hospital sites now under agreement, doubling participating centers
  • Pilot trial to enroll up to 30 adult DDD patients undergoing TLIF
  • Primary endpoints include fusion success at 12 and 24 months and Oswestry Disability Index change
  • Study design reviewed by FDA’s Division of Orthopedic Devices in a pre-submission
  • Company reports cash runway through Q2 2027
  • rhNELL-1 has a 32-month validated shelf life

Negative

  • None.

Market Context

On Aug 12, the rhNELL-1 shelf-life milestone carried a 28.61% negative supplied 24-hour reaction des...
Analysis

On Aug 12, the rhNELL-1 shelf-life milestone carried a 28.61% negative supplied 24-hour reaction despite supporting the same NB1 program; this update adds sites but reports no efficacy results.

Key Figures

Participating hospital sites: 6 sites Expected enrollment completion: Year-end 2026 Planned subjects: Up to 30 adult subjects +3 more
Participating hospital sites
6 sites
Australia pilot clinical trial
Expected enrollment completion
Year-end 2026
First-in-human pilot study
Planned subjects
Up to 30 adult subjects
Pilot study in degenerative disc disease
Primary endpoint follow-up
12 and 24 months
Following surgery
Cash runway
Through Q2 2027
Funding NB1 clinical program
Validated rhNELL-1 shelf life
32 months
Recently announced product milestone

Historical Context

2 past events · Latest: Aug 12
2 events
  1. Aug 12

    Shelf-life milestone

    24h Move
    -28.6%

    Validated rhNELL-1 shelf life extended to 32 months for pivotal-study preparation

  2. Jul 07

    Private placement

    24h Move
    -8.8%

    Private placement provided clinical-trial funding and potential additional warrant proceeds

24h Move is the share-price change in the day after each event; other market factors may also have contributed.

Key Terms

demineralized bone matrix, transforaminal lumbar interbody fusion, oswestry disability index, pre-submission
4 terms
demineralized bone matrix medical
"combines the recombinant human NELL-1 protein (rhNELL-1) with demineralized bone matrix"
Demineralized bone matrix is human bone that has had its mineral components removed, leaving a protein-based scaffold that helps the body grow new bone. Think of it as a biological framework or scaffold that surgeons use in orthopedics and dental procedures to support healing and tissue regrowth. For investors, it matters because its clinical utility, regulatory clearance, manufacturing quality, and adoption by surgeons affect product demand, company revenue, and reimbursement dynamics in the medical products market.
transforaminal lumbar interbody fusion medical
"undergoing transforaminal lumbar interbody fusion (TLIF)"
A transforaminal lumbar interbody fusion is a type of spine surgery that stabilizes two adjacent lower-back vertebrae by reaching the disc space through the natural side opening where spinal nerves exit, removing damaged disc material, and placing bone graft and an implant to fuse the bones together. Investors care because the procedure drives demand for spinal implants, surgical devices, hospital services, rehabilitation and insurance payments, so trends in its use, outcomes and reimbursement affect medical device makers, hospitals and payers much like sales trends affect any product line.
oswestry disability index medical
"the Oswestry Disability Index, a standard measure of functional disability"
A standardized questionnaire used to measure how much back pain limits a person’s everyday activities, like lifting, walking or sleeping; patients answer a set of simple questions and receive a score that reflects their level of disability. Investors pay attention because changes in this score are often used in clinical trials and regulatory reviews to demonstrate whether a treatment or device meaningfully improves patients’ quality of life, which can affect approval, market adoption and commercial value.
pre-submission regulatory
"previously reviewed the study design in a pre-submission"
A pre-submission is an organized, formal request by a company to a regulatory authority asking for feedback on planned data, study designs, or application content before the company files an official regulatory submission. It’s like asking a referee to review a playbook before the game starts: the regulator can point out gaps or clarify expectations. Investors care because such early input can reduce regulatory uncertainty, shorten review time, and affect the odds and timing of approval-related milestones.

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Addition of three sites strengthens expectation to complete enrollment by year-end 2026

BURLINGTON, MA, Sept. 08, 2026 (GLOBE NEWSWIRE) -- Bone Biologics Corporation (Nasdaq: BBLG, BBLGW) (“Bone Biologics” or the “Company”), a developer of orthobiologic products for spine fusion markets, today announced that six hospital sites in Australia are now under agreement to participate in its first-in-human pilot clinical trial of NB1, the Company’s rhNELL-1-based bone graft product for spinal fusion. The expanded network doubles the number of participating centers and is expected to accelerate patient screening and surgical scheduling, strengthening the Company’s expectation to complete enrollment by year-end 2026.

NB1, the Company’s lead product candidate, combines the recombinant human NELL-1 protein (rhNELL-1) with demineralized bone matrix for use in spinal procedures.

“We believe the additional hospital sites in our study meaningfully increases our access to qualified patients and reduces our reliance on patient flow at any individual center,” said Bone Biologics CEO Jeff Frelick. “This expansion strengthens our anticipation of reaching full enrollment by the end 2026 and brings us closer to the 12-month follow-up data.”

Study design and endpoints

The pilot study is a multicenter, prospective, parallel-group, randomized trial conducted in Australia that evaluates the safety and preliminary effectiveness of NB1 in up to 30 adult subjects with degenerative disc disease (DDD) undergoing transforaminal lumbar interbody fusion (TLIF). Inclusion criteria include patients with DDD at one level from L2-S1 who may also have up to Grade 1 spondylolisthesis or Grade 1 retrolisthesis at the involved level. Primary clinical endpoints include fusion success at 12 and 24 months following surgery, and change from baseline in the Oswestry Disability Index, a standard measure of functional disability. The U.S. Food and Drug Administration’s Division of Orthopedic Devices previously reviewed the study design in a pre-submission, and these pilot study findings are intended to support progression to a pivotal clinical study in the United States.

“Supported by our cash runway through the second quarter of 2027 and our recently announced 32-month validated shelf life for rhNELL-1, we remain focused on disciplined execution of the NB1 clinical program as we prepare for a potential pivotal study,” added Frelick.

About Bone Biologics

Bone Biologics was founded to pursue regenerative medicine for bone. The Company is undertaking work with select strategic partners that builds on the preclinical research of the NELL-1 protein. Bone Biologics is focusing development efforts for its bone graft substitute product on bone regeneration in spinal fusion procedures, while additionally having rights to trauma and osteoporosis applications. For more information, please visit www.bonebiologics.com.

Forward-Looking Statements

Certain statements contained in this press release, including, without limitation, statements regarding the number and participation of hospital sites in the Company’s pilot clinical study, the expected timing of completion of patient enrollment, the timing, implementation and success of the Company’s pilot clinical study and any interim updates, the Company’s operating runway, the Company’s development of rhNELL-1 and achievement of operational milestones, the ability of the Company’s lead product candidate NB1 to provide rapid, specific and guided control over bone regeneration and show fusion success in humans, the ability of NB1 to compete in global markets, and the Company’s progression toward a potential pivotal clinical study, as well as statements containing the words “may,” “anticipate,” “believe,” “continue,” “expect,” “focus,” “intend,” “will,” and words of similar import, constitute “forward-looking statements” within the meaning of the Private Securities Litigation Reform Act of 1995. Such forward-looking statements involve both known and unknown risks and uncertainties. The Company’s actual results may differ materially from those anticipated in its forward-looking statements as a result of a number of factors, including, but not limited to, market and other conditions and risks generally associated with an undercapitalized developing company, as well as the risks contained under “Risk Factors” and “Management’s Discussion and Analysis of Financial Condition and Results of Operations” in the Company’s Annual Report on Form 10-K for the year ended December 31, 2025 and the Company’s other filings with the Securities and Exchange Commission. Except as required by applicable law, we undertake no obligation to revise or update any forward-looking statements to reflect any event or circumstance that may arise after the date hereof.

Contacts
CORE IR
(212) 655-0924
investors@bonebiologics.com


FAQ

What is NB1 and how is it intended to be used?

NB1 is Bone Biologics’ lead product candidate, a bone graft that combines recombinant human NELL-1 protein (rhNELL-1) with demineralized bone matrix. It is intended for use in spinal fusion procedures.

What are the key design features of the NB1 pilot clinical study?

The pilot is a multicenter, prospective, parallel-group, randomized trial in Australia. It will evaluate the safety and preliminary effectiveness of NB1 in up to 30 adult subjects with degenerative disc disease undergoing transforaminal lumbar interbody fusion (TLIF).

Which patients are eligible for enrollment in the NB1 pilot trial?

Inclusion criteria cover adults with degenerative disc disease at one level from L2–S1, who may also have up to Grade 1 spondylolisthesis or Grade 1 retrolisthesis at the involved level.

What are the primary clinical endpoints of the NB1 trial?

Primary endpoints include fusion success at 12 and 24 months after surgery and change from baseline in the Oswestry Disability Index, a standard measure of functional disability.

How has the FDA been involved with the NB1 clinical program so far?

The FDA’s Division of Orthopedic Devices has previously reviewed the pilot study design in a pre-submission. The company intends for the pilot findings to support progression to a pivotal clinical study in the United States.

How is Bone Biologics supporting execution of the NB1 program operationally and financially?

The company highlights that it is supported by a cash runway through the second quarter of 2027 and a 32-month validated shelf life for rhNELL-1, and states that it remains focused on disciplined execution of the NB1 clinical program as it prepares for a potential pivotal study.

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