BillionToOne, Inc. received a Schedule 13G reporting that venture investor NeoTribe-related funds and managing member Krishna Kittu Kolluri together beneficially own a significant stake in the company’s Class A common stock.
The filing shows NTV I holding 2,710,286 shares (6.6%), NTIF I holding 1,297,799 shares (3.1%), and NT SPV I holding 242,216 shares (0.6%), among other related entities. Aggregating these positions, Kolluri is reported to beneficially own 4,359,505 shares, representing 10.6% of the Class A common stock, with sole voting and dispositive power reported through various NeoTribe general partner and managing member roles. All ownership percentages are based on 41,216,110 shares of Class A common stock outstanding as of December 5, 2025, as stated in the company’s Form 10-Q.
Positive
None.
Negative
None.
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
SCHEDULE 13G
UNDER THE SECURITIES EXCHANGE ACT OF 1934
BillionToOne, Inc.
(Name of Issuer)
Class A Common Stock, par value $0.00001 per share
(Title of Class of Securities)
090168105
(CUSIP Number)
11/07/2025
(Date of Event Which Requires Filing of this Statement)
Check the appropriate box to designate the rule pursuant to which this Schedule is filed:
Rule 13d-1(b)
Rule 13d-1(c)
Rule 13d-1(d)
SCHEDULE 13G
CUSIP No.
090168105
1
Names of Reporting Persons
NeoTribe Ventures I, L.P. ("NTV I")
2
Check the appropriate box if a member of a Group (see instructions)
(a)
(b)
3
Sec Use Only
4
Citizenship or Place of Organization
DELAWARE
Number of Shares Beneficially Owned by Each Reporting Person With:
5
Sole Voting Power
2,710,286.00
6
Shared Voting Power
0.00
7
Sole Dispositive Power
2,710,286.00
8
Shared Dispositive Power
0.00
9
Aggregate Amount Beneficially Owned by Each Reporting Person
2,710,286.00
10
Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)
11
Percent of class represented by amount in row (9)
6.6 %
12
Type of Reporting Person (See Instructions)
PN
Comment for Type of Reporting Person: Note to Rows 5 and 7: NeoTribe Partners I, LLC ("NTP I"), the general partner of NTV I, may be deemed to have sole voting and dispositive power over these shares, and Kolluri, the managing member of NTP I, may be deemed to have sole voting and dispositive power over these shares.
Note to Row 6: See response to Row 5.
Note to Row 8: See response to Row 7.
Note to Row 11: This percentage is calculated based upon 41,216,110 shares of Class A Common Stock, par value $0.00001 per share ("Common Stock") outstanding as of December 5, 2025, as set forth in the quarterly report on Form 10-Q of BillionToOne, Inc. (the "Issuer") for the quarter ended September 30, 2025, filed with the United States Securities and Exchange Commission (the "Commission") on December 10, 2025 (the "Form 10-Q").
SCHEDULE 13G
CUSIP No.
090168105
1
Names of Reporting Persons
NeoTribe Associates I, L.P. ("NTA I")
2
Check the appropriate box if a member of a Group (see instructions)
(a)
(b)
3
Sec Use Only
4
Citizenship or Place of Organization
DELAWARE
Number of Shares Beneficially Owned by Each Reporting Person With:
5
Sole Voting Power
109,204.00
6
Shared Voting Power
0.00
7
Sole Dispositive Power
109,204.00
8
Shared Dispositive Power
0.00
9
Aggregate Amount Beneficially Owned by Each Reporting Person
109,204.00
10
Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)
11
Percent of class represented by amount in row (9)
0.3 %
12
Type of Reporting Person (See Instructions)
PN
Comment for Type of Reporting Person: Note to Rows 5 and 7: All shares are held of record by NTV I as nominee for NTA I. NTP I, the general partner of NTA I, may be deemed to have sole voting and dispositive power over these shares, and Kolluri, the managing member of NTP I, may be deemed to have sole voting and dispositive power over these shares.
Note to Row 6: See response to Row 5.
Note to Row 8: See response to Row 7.
Note to Row 11: This percentage is calculated based upon 41,216,110 shares of Common Stock outstanding as of December 5, 2025, as set forth in the Form 10-Q.
SCHEDULE 13G
CUSIP No.
090168105
1
Names of Reporting Persons
NeoTribe Partners I, LLC ("NTP I")
2
Check the appropriate box if a member of a Group (see instructions)
(a)
(b)
3
Sec Use Only
4
Citizenship or Place of Organization
DELAWARE
Number of Shares Beneficially Owned by Each Reporting Person With:
5
Sole Voting Power
2,819,490.00
6
Shared Voting Power
0.00
7
Sole Dispositive Power
2,819,490.00
8
Shared Dispositive Power
0.00
9
Aggregate Amount Beneficially Owned by Each Reporting Person
2,819,490.00
10
Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)
11
Percent of class represented by amount in row (9)
6.8 %
12
Type of Reporting Person (See Instructions)
OO
Comment for Type of Reporting Person: Note to Rows 5 and 7: Consists of 2,710,286 shares held of record by NTV I for itself and 109,204 shares held of record by NTV I as nominee for NTA I. NTP I, the general partner of NTV I and NTA I, may be deemed to have sole voting and dispositive power over these shares, and Kolluri, the managing member of NTP I, may be deemed to have sole voting and dispositive power over these shares.
Note to Row 6: See response to Row 5.
Note to Row 8: See response to Row 7.
Note to Row 11: This percentage is calculated based upon 41,216,110 shares of Common Stock outstanding as of December 5, 2025, as set forth in the Form 10-Q.
SCHEDULE 13G
CUSIP No.
090168105
1
Names of Reporting Persons
Neotribe Ignite Fund I, L.P. ("NTIF I")
2
Check the appropriate box if a member of a Group (see instructions)
(a)
(b)
3
Sec Use Only
4
Citizenship or Place of Organization
DELAWARE
Number of Shares Beneficially Owned by Each Reporting Person With:
5
Sole Voting Power
1,297,799.00
6
Shared Voting Power
0.00
7
Sole Dispositive Power
1,297,799.00
8
Shared Dispositive Power
0.00
9
Aggregate Amount Beneficially Owned by Each Reporting Person
1,297,799.00
10
Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)
11
Percent of class represented by amount in row (9)
3.1 %
12
Type of Reporting Person (See Instructions)
PN
Comment for Type of Reporting Person: Note to Rows 5 and 7: Neotribe Ignite Partners I, LLC ("NTIP I"), the general partner of NTIF I, may be deemed to have sole voting and dispositive power over these shares, and Kolluri, the managing member of NTIP I, may be deemed to have sole voting and dispositive power over these shares.
Note to Row 6: See response to Row 5.
Note to Row 8: See response to Row 7.
Note to Row 11: This percentage is calculated based upon 41,216,110 shares of Common Stock outstanding as of December 5, 2025, as set forth in the Form 10-Q.
SCHEDULE 13G
CUSIP No.
090168105
1
Names of Reporting Persons
Neotribe Ignite Partners I, LLC ("NTIP I")
2
Check the appropriate box if a member of a Group (see instructions)
(a)
(b)
3
Sec Use Only
4
Citizenship or Place of Organization
DELAWARE
Number of Shares Beneficially Owned by Each Reporting Person With:
5
Sole Voting Power
1,297,799.00
6
Shared Voting Power
0.00
7
Sole Dispositive Power
1,297,799.00
8
Shared Dispositive Power
0.00
9
Aggregate Amount Beneficially Owned by Each Reporting Person
1,297,799.00
10
Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)
11
Percent of class represented by amount in row (9)
3.1 %
12
Type of Reporting Person (See Instructions)
OO
Comment for Type of Reporting Person: Note to Rows 5 and 7: Shares held of record by NTIF I. NTIP I, the general partner of NTIF I, may be deemed to have sole voting and dispositive power over these shares, and Kolluri, the managing member of NTIF I, may be deemed to have sole voting and dispositive power over these shares.
Note to Row 6: See response to Row 5.
Note to Row 8: See response to Row 7.
Note to Row 11: This percentage is calculated based upon 41,216,110 shares of Common Stock outstanding as of December 5, 2025, as set forth in the Form 10-Q.
SCHEDULE 13G
CUSIP No.
090168105
1
Names of Reporting Persons
Neotribe SPV I BTO, LLC ("NT SPV I")
2
Check the appropriate box if a member of a Group (see instructions)
(a)
(b)
3
Sec Use Only
4
Citizenship or Place of Organization
DELAWARE
Number of Shares Beneficially Owned by Each Reporting Person With:
5
Sole Voting Power
242,216.00
6
Shared Voting Power
0.00
7
Sole Dispositive Power
242,216.00
8
Shared Dispositive Power
0.00
9
Aggregate Amount Beneficially Owned by Each Reporting Person
242,216.00
10
Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)
11
Percent of class represented by amount in row (9)
0.6 %
12
Type of Reporting Person (See Instructions)
OO
Comment for Type of Reporting Person: Note to Rows 5 and 7: Neotribe Partners SPV I BTO, LLC ("NTP SPV I"), the managing member of NT SPV I, may be deemed to have sole voting and dispositive power over these shares, and Kolluri, the managing member of NTP SPV I, may be deemed to have sole voting and dispositive power over these shares.
Note to Row 6: See response to Row 5.
Note to Row 8: See response to Row 7.
Note to Row 11: This percentage is calculated based upon 41,216,110 shares of Common Stock outstanding as of December 5, 2025, as set forth in the Form 10-Q.
SCHEDULE 13G
CUSIP No.
090168105
1
Names of Reporting Persons
Neotribe Partners SPV I BTO, LLC ("NTP SPV I")
2
Check the appropriate box if a member of a Group (see instructions)
(a)
(b)
3
Sec Use Only
4
Citizenship or Place of Organization
DELAWARE
Number of Shares Beneficially Owned by Each Reporting Person With:
5
Sole Voting Power
242,216.00
6
Shared Voting Power
0.00
7
Sole Dispositive Power
242,216.00
8
Shared Dispositive Power
0.00
9
Aggregate Amount Beneficially Owned by Each Reporting Person
242,216.00
10
Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)
11
Percent of class represented by amount in row (9)
0.6 %
12
Type of Reporting Person (See Instructions)
OO
Comment for Type of Reporting Person: Note to Row 5: Shares held of record by NT SPV I. NTP SPV I, the managing member of NT SPV I, may be deemed to have sole voting and dispositive power over these shares, and Kolluri, the managing member of NT SPV I, may be deemed to have sole voting and dispositive power over these shares.
Note to Row 6: See response to Row 5.
Note to Row 8: See response to Row 7.
Note to Row 11: This percentage is calculated based upon 41,216,110 shares of Common Stock outstanding as of December 5, 2025, as set forth in the Form 10-Q.
SCHEDULE 13G
CUSIP No.
090168105
1
Names of Reporting Persons
Krishna Kittu Kolluri ("Kolluri")
2
Check the appropriate box if a member of a Group (see instructions)
(a)
(b)
3
Sec Use Only
4
Citizenship or Place of Organization
UNITED STATES
Number of Shares Beneficially Owned by Each Reporting Person With:
5
Sole Voting Power
4,359,505.00
6
Shared Voting Power
0.00
7
Sole Dispositive Power
4,359,505.00
8
Shared Dispositive Power
0.00
9
Aggregate Amount Beneficially Owned by Each Reporting Person
4,359,505.00
10
Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)
11
Percent of class represented by amount in row (9)
10.6 %
12
Type of Reporting Person (See Instructions)
IN
Comment for Type of Reporting Person: Note to Rows 5 and 7: Consists of 2,710,286 shares held of record by NTV I for itself, 109,204 shares held of record by NTV I as nominee for NTA I, 1,297,799 shares held of record by NTIF I and 242,216 shares held of record by NT SPV I. NTP I, the general partner of NTV I and NTA I, may be deemed to have sole voting and dispositive power over the shares held of record by NTV I for itself and as nominee for NTA I. NTIP I, the general partner of NTIF I, may be deemed to have sole voting and dispositive power over the shares held of record by NTIF I. NTP SPV I, the managing member of NT SPV I, may be deemed to have sole voting and dispositive power over the shares held of record by NT SPV I. Kolluri, a director of the Issuer and the managing member of NTP I, NTIP I and NTP SPV I may be deemed to have sole voting and dispositive power over the shares held of record by NTV I for itself and as nominee for NTA I, NTIF I and NT SPV I, respectively.
Note to Row 6: See response to Row 5.
Note to Row 8: See response to Row 7.
Note to Row 11: This percentage is calculated based upon 41,216,110 shares of Common Stock outstanding as of December 5, 2025, as set forth in the Form 10-Q.
SCHEDULE 13G
Item 1.
(a)
Name of issuer:
BillionToOne, Inc.
(b)
Address of issuer's principal executive offices:
1035 O'Brien Drive, Menlo Park, CA 94025
Item 2.
(a)
Name of person filing:
This joint statement on Schedule 13G is being filed by NeoTribe Ventures I, L.P. ("NTV I"), NeoTribe Associates I, L.P. ("NTA I"), NeoTribe Partners I, LLC ("NTP I"), Neotribe Ignite Fund I, L.P. ("NTIF I"), Neotribe Ignite Partners I, LLC ("NTIP I"), Neotribe SPV I BTO, LLC ("NT SPV I"), Neotribe Partners SPV I BTO, LLC ("NTP SPV I") and Krishna Kolluri ("Kolluri"). The foregoing entities and individuals are collectively referred to as the "Reporting Persons."
(b)
Address or principal business office or, if none, residence:
c/o NeoTribe Management Company, LLC
1300 El Camino Real, Suite 100
Menlo Park, CA 94025
(c)
Citizenship:
See Row 4 of cover page for each Reporting Person.
(d)
Title of class of securities:
Class A Common Stock, par value $0.00001 per share
(e)
CUSIP No.:
090168105
Item 3.
If this statement is filed pursuant to §§ 240.13d-1(b) or 240.13d-2(b) or (c), check whether the person filing is a:
(a)
Broker or dealer registered under section 15 of the Act (15 U.S.C. 78o);
(b)
Bank as defined in section 3(a)(6) of the Act (15 U.S.C. 78c);
(c)
Insurance company as defined in section 3(a)(19) of the Act (15 U.S.C. 78c);
(d)
Investment company registered under section 8 of the Investment Company Act of 1940 (15 U.S.C. 80a-8);
(e)
An investment adviser in accordance with § 240.13d-1(b)(1)(ii)(E);
(f)
An employee benefit plan or endowment fund in accordance with § 240.13d-1(b)(1)(ii)(F);
(g)
A parent holding company or control person in accordance with § 240.13d-1(b)(1)(ii)(G);
(h)
A savings associations as defined in Section 3(b) of the Federal Deposit Insurance Act (12 U.S.C. 1813);
(i)
A church plan that is excluded from the definition of an investment company under section 3(c)(14) of the Investment Company Act of 1940 (15 U.S.C. 80a-3);
(j)
A non-U.S. institution in accordance with § 240.13d-1(b)(1)(ii)(J). If filing as a non-U.S. institution in accordance with § 240.13d-1(b)(1)(ii)(J),
please specify the type of institution:
(k)
Group, in accordance with Rule 240.13d-1(b)(1)(ii)(K).
Item 4.
Ownership
(a)
Amount beneficially owned:
See Row 9 of cover page for each Reporting Person.
(b)
Percent of class:
See Row 11 of cover page for each Reporting Person.
(c)
Number of shares as to which the person has:
(i) Sole power to vote or to direct the vote:
See Row 5 of cover page for each Reporting Person.
(ii) Shared power to vote or to direct the vote:
See Row 6 of cover page for each Reporting Person.
(iii) Sole power to dispose or to direct the disposition of:
See Row 7 of cover page for each Reporting Person.
(iv) Shared power to dispose or to direct the disposition of:
See Row 8 of cover page for each Reporting Person.
Item 5.
Ownership of 5 Percent or Less of a Class.
Not Applicable
Item 6.
Ownership of more than 5 Percent on Behalf of Another Person.
If any other person is known to have the right to receive or the power to direct the receipt of dividends from, or the proceeds from the sale of, such securities, a statement to that effect should be included in response to this item and, if such interest relates to more than 5 percent of the class, such person should be identified. A listing of the shareholders of an investment company registered under the Investment Company Act of 1940 or the beneficiaries of employee benefit plan, pension fund or endowment fund is not required.
Under certain circumstances set forth in the limited partnership agreements of NTV I, NTA I and NTIF I, and the limited liability company agreement of NTP I, NTIP I, NT SPV I and NTP SPV I, the partners or members, as the case may be, of each of such entities may be deemed to have the right to receive dividends from, or the proceeds from the sale of, shares of the Issuer directly or indirectly owned by each such entity of which they are a partner or member.
Item 7.
Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on by the Parent Holding Company or Control Person.
Not Applicable
Item 8.
Identification and Classification of Members of the Group.
Not Applicable
Item 9.
Notice of Dissolution of Group.
Not Applicable
Item 10.
Certifications:
Not Applicable
SIGNATURE
After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.
NeoTribe Ventures I, L.P. ("NTV I")
Signature:
/s/ Krishna Kolluri
Name/Title:
Managing Member of NeoTribe Partners I, LLC, the General Partner of NeoTribe Ventures I, L.P.
Date:
02/13/2026
NeoTribe Associates I, L.P. ("NTA I")
Signature:
/s/ Krishna Kolluri
Name/Title:
Managing Member of NeoTribe Partners I, LLC, the General Partner of NeoTribe Associates I, L.P.
Date:
02/13/2026
NeoTribe Partners I, LLC ("NTP I")
Signature:
/s/ Krishna Kolluri
Name/Title:
Managing Member
Date:
02/13/2026
Neotribe Ignite Fund I, L.P. ("NTIF I")
Signature:
/s/ Krishna Kolluri
Name/Title:
Managing Member of Neotribe Ignite Partners I, LLC, the General Partner of Neotribe Ignite Fund I, L.P.
Date:
02/13/2026
Neotribe Ignite Partners I, LLC ("NTIP I")
Signature:
/s/ Krishna Kolluri
Name/Title:
Managing Member
Date:
02/13/2026
Neotribe SPV I BTO, LLC ("NT SPV I")
Signature:
/s/ Krishna Kolluri
Name/Title:
Managing Member of Neotribe Partners SPV I BTO, LLC, the Managing Member of Neotribe SPV I BTO, LLC
What stake in BillionToOne (BLLN) does Krishna Kolluri report on this Schedule 13G?
The Schedule 13G reports that Krishna Kittu Kolluri beneficially owns 4,359,505 shares of BillionToOne Class A common stock, representing 10.6% of the class. This ownership is held through several NeoTribe funds and special purpose vehicles over which Kolluri may exercise control.
Which NeoTribe entities are reporting beneficial ownership in BillionToOne (BLLN)?
The filing lists NeoTribe Ventures I, L.P., NeoTribe Associates I, L.P., NeoTribe Partners I, LLC, Neotribe Ignite Fund I, L.P., Neotribe Ignite Partners I, LLC, Neotribe SPV I BTO, LLC, and Neotribe Partners SPV I BTO, LLC as reporting persons, together with Krishna Kittu Kolluri as an individual.
How many BillionToOne (BLLN) shares does NeoTribe Ventures I, L.P. hold?
NeoTribe Ventures I, L.P. is reported as beneficially owning 2,710,286 shares of BillionToOne Class A common stock. This position represents 6.6% of the outstanding class, with sole voting and dispositive power attributed to NeoTribe Ventures I, L.P. in the cover page data.
On what share count are the ownership percentages for BillionToOne (BLLN) based?
All reported ownership percentages use 41,216,110 BillionToOne Class A common shares outstanding as the base. This outstanding share figure is taken from BillionToOne’s Form 10-Q for the quarter ended September 30, 2025, which states shares outstanding as of December 5, 2025.
What is the difference between NTIF I and NT SPV I holdings in BillionToOne (BLLN)?
Neotribe Ignite Fund I, L.P. (NTIF I) beneficially owns 1,297,799 shares, or 3.1% of the class. Neotribe SPV I BTO, LLC (NT SPV I) holds 242,216 shares, or 0.6%. Both entities report sole voting and dispositive power through their respective managing general partner entities.
What type of SEC filing is this for BillionToOne (BLLN) and what does it indicate?
This is a Schedule 13G filing, which reports passive beneficial ownership of more than 5% of a registered class of equity securities. It indicates that the listed NeoTribe entities and Krishna Kolluri collectively hold a sizable, disclosed position in BillionToOne’s Class A common stock.
