[Form 4] Cardio Diagnostics Holdings, Inc. Insider Trading Activity

Rhea-AI Filing Summary

Paul Burton, a director of Cardio Diagnostics Holdings, Inc. (CDIO), exercised stock options on 09/30/2025 to acquire 1,559 shares at an exercise price of $4.01 per share. The options are exercisable immediately and expire on 09/30/2035. Following the reported transaction, Mr. Burton beneficially owns 4,953 shares of common stock. The Form 4 was filed via signature by Elisa Luqman as attorney-in-fact on 10/03/2025. The filing identifies the reporting person as a director and shows the ownership form as direct.

SEC Form 4

FORM 4

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934
or Section 30(h) of the Investment Company Act of 1940

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Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

1. Name and Address of Reporting Person^*

Burton Paul Frederick

(Last) (First) (Middle)

C/O CARDIO DIAGNOSTICS HOLDINGS INC.

311 W. SUPERIOR ST. SUITE 444

(Street)

CHICAGO IL 60654

(City) (State) (Zip)

2. Issuer Name and Ticker or Trading Symbol
Cardio Diagnostics Holdings, Inc. [ CDIO ]

5. Relationship of Reporting Person(s) to Issuer
(Check all applicable)

X	Director		10% Owner
	Officer (give title below)		Other (specify below)

3. Date of Earliest Transaction (Month/Day/Year)
09/30/2025

4. If Amendment, Date of Original Filed (Month/Day/Year)

6. Individual or Joint/Group Filing (Check Applicable Line)

X	Form filed by One Reporting Person
	Form filed by More than One Reporting Person

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned
1. Title of Security (Instr. 3)	2. Transaction Date (Month/Day/Year)	2A. Deemed Execution Date, if any (Month/Day/Year)	3. Transaction Code (Instr. 8)		4. Securities Acquired (A) or Disposed Of (D) (Instr. 3, 4 and 5)			5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4)	6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4)	7. Nature of Indirect Beneficial Ownership (Instr. 4)
			Code	V	Amount	(A) or (D)	Price

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned (e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security (Instr. 3)	2. Conversion or Exercise Price of Derivative Security	3. Transaction Date (Month/Day/Year)	3A. Deemed Execution Date, if any (Month/Day/Year)	4. Transaction Code (Instr. 8)		5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5)		6. Date Exercisable and Expiration Date (Month/Day/Year)		7. Title and Amount of Securities Underlying Derivative Security (Instr. 3 and 4)		8. Price of Derivative Security (Instr. 5)	9. Number of derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4)	10. Ownership Form: Direct (D) or Indirect (I) (Instr. 4)	11. Nature of Indirect Beneficial Ownership (Instr. 4)
				Code	V	(A)	(D)	Date Exercisable	Expiration Date	Title	Amount or Number of Shares
Stock Options (Right to Buy)	$4.01	09/30/2025		A		1,559		09/30/2025	09/30/2035	Common Stock	1,559	$4.01	4,953	D

Explanation of Responses:

	/s/ Elisa Luqman as attorney-in-fact for Paul Burton	10/03/2025
	** Signature of Reporting Person	Date
Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
* If the form is filed by more than one reporting person, see Instruction 4 (b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
Note: File three copies of this Form, one of which must be manually signed. If space is insufficient, see Instruction 6 for procedure.
Persons who respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB Number.

FAQ

What insider transaction did Cardio Diagnostics (CDIO) disclose on this Form 4?

The filing shows director Paul Burton exercised stock options to acquire 1,559 shares on 09/30/2025 at an exercise price of $4.01.

How many CDIO shares does Paul Burton own after the transaction?

After the reported exercise, Mr. Burton beneficially owns 4,953 shares of CDIO common stock.

What are the terms of the exercised options disclosed for CDIO?

The disclosed options have an exercise price of $4.01, were exercised on 09/30/2025, and expire on 09/30/2035.

Who signed the Form 4 for Paul Burton and when was it signed?

The Form 4 is signed by Elisa Luqman as attorney-in-fact for Paul Burton on 10/03/2025.

Does the Form 4 indicate whether Paul Burton is an officer or director of CDIO?

Yes, the form checks the box indicating the reporting person is a Director of Cardio Diagnostics Holdings, Inc.