[Form 3] enGene Holdings Inc. Warrants Initial Statement of Beneficial Ownership

Insider Trade Summary

3 transactions reported

Mixed 3 txns

Insider Boyd Matthew Ross

Role Chief Regulatory Officer

Type	Security	Shares	Price	Value
holding	Stock Option (Right to Buy)	--	--	--
holding	Stock Option (Right to Buy)	--	--	--
holding	Common Shares	--	--	--

Holdings After Transaction: Stock Option (Right to Buy) — 119,000 shares (Direct); Common Shares — 7,500 shares (Direct)

Footnotes (2)

1. F1. Non-qualified stock option grant awarded as an inducement award outside of the Amended and Restated enGene Holdings Inc. 2023 Incentive Equity Plan in accordance with NASDAQ Listing Rule 5635(c)(4). This option vests at 25% on September 16, 2025, which is the first anniversary of the employment commencement date, with the remaining portion to vest monthly over the next three years, subject to the reporting person's continued service.
2. F2. This option vests in equal monthly installments over forty-eight (48) months on the last day of each month commencing January 29, 2025, subject to the reporting person's continued service.

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Accepted by SEC EDGAR 07/14/2025 - 04:09 PM Learn about SEC filing dates

SEC Form 3

FORM 3

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

INITIAL STATEMENT OF BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934
or Section 30(h) of the Investment Company Act of 1940

OMB APPROVAL

OMB Number:			3235-0104
Estimated average burden
hours per response:			0.5

1. Name and Address of Reporting Person^*

Boyd Matthew Ross

(Last) (First) (Middle)

C/O 4868 RUE LEVY, SUITE 220

(Street)

SAINT-LAURENT A8 H4R 2P1

(City) (State) (Zip)

2. Date of Event Requiring Statement (Month/Day/Year)
07/08/2025

3. Issuer Name and Ticker or Trading Symbol
enGene Holdings Inc. [ ENGN ]

4. Relationship of Reporting Person(s) to Issuer
(Check all applicable)

	Director		10% Owner
X	Officer (give title below)		Other (specify below)
	Chief Regulatory Officer

5. If Amendment, Date of Original Filed (Month/Day/Year)

6. Individual or Joint/Group Filing (Check Applicable Line)

X	Form filed by One Reporting Person
	Form filed by More than One Reporting Person

Table I - Non-Derivative Securities Beneficially Owned
1. Title of Security (Instr. 4)	2. Amount of Securities Beneficially Owned (Instr. 4)	3. Ownership Form: Direct (D) or Indirect (I) (Instr. 5)	4. Nature of Indirect Beneficial Ownership (Instr. 5)
Common Shares	7,500	D

Table II - Derivative Securities Beneficially Owned (e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security (Instr. 4)	2. Date Exercisable and Expiration Date (Month/Day/Year)		3. Title and Amount of Securities Underlying Derivative Security (Instr. 4)		4. Conversion or Exercise Price of Derivative Security	5. Ownership Form: Direct (D) or Indirect (I) (Instr. 5)	6. Nature of Indirect Beneficial Ownership (Instr. 5)
	Date Exercisable	Expiration Date	Title	Amount or Number of Shares
Stock Option (Right to Buy)	(1)	09/30/2034	Common Shares	68,000	$6.6	D
Stock Option (Right to Buy)	(2)	01/29/2035	Common Shares	51,000	$7.39	D

Explanation of Responses:

1. Non-qualified stock option grant awarded as an inducement award outside of the Amended and Restated enGene Holdings Inc. 2023 Incentive Equity Plan in accordance with NASDAQ Listing Rule 5635(c)(4). This option vests at 25% on September 16, 2025, which is the first anniversary of the employment commencement date, with the remaining portion to vest monthly over the next three years, subject to the reporting person's continued service.

2. This option vests in equal monthly installments over forty-eight (48) months on the last day of each month commencing January 29, 2025, subject to the reporting person's continued service.

Remarks:

Exhibit 24 - Power of Attorney

	/s/ Lee Giguere, as attorney-in-fact for the Reporting Person	07/14/2025
	** Signature of Reporting Person	Date
Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
* If the form is filed by more than one reporting person, see Instruction 5 (b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
Note: File three copies of this Form, one of which must be manually signed. If space is insufficient, see Instruction 6 for procedure.
Persons who respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB Number.