William Blair reports 9.7% Twist Bioscience (TWST) stake in 13G/A

Rhea-AI Filing Summary

William Blair Investment Management, LLC has filed Amendment No. 1 to a Schedule 13G reporting its ownership in Twist Bioscience Corp. As of the event date of 12/31/2025, the firm beneficially owned 5,954,429 shares of Twist Bioscience common stock, representing 9.7% of the outstanding class.

The filer reports sole voting power over 5,459,833 shares and sole dispositive power over 5,954,429 shares, with no shared voting or dispositive power. The securities are stated to be held in the ordinary course of business and not for the purpose of changing or influencing control of Twist Bioscience.

SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

SCHEDULE 13G

UNDER THE SECURITIES EXCHANGE ACT OF 1934

(Amendment No. 1)

Twist Bioscience Corp

(Name of Issuer)

Common Stock

(Title of Class of Securities)

90184D100

(CUSIP Number)

12/31/2025

(Date of Event Which Requires Filing of this Statement)

Check the appropriate box to designate the rule pursuant to which this Schedule is filed:

Rule 13d-1(b)

Rule 13d-1(c)

Rule 13d-1(d)

SCHEDULE 13G

CUSIP No. 90184D100

1

Names of Reporting Persons

WILLIAM BLAIR INVESTMENT MANAGEMENT, LLC

2

Check the appropriate box if a member of a Group (see instructions)

(a)
(b)

3

Sec Use Only

4

Citizenship or Place of Organization

DELAWARE

Number of Shares Beneficially Owned by Each Reporting Person With:

5	Sole Voting Power 5,459,833.00
6	Shared Voting Power 0.00
7	Sole Dispositive Power 5,954,429.00
8	Shared Dispositive Power 0.00

9

Aggregate Amount Beneficially Owned by Each Reporting Person

5,954,429.00

10

Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)

11

Percent of class represented by amount in row (9)

9.7 %

12

Type of Reporting Person (See Instructions)

IA

SCHEDULE 13G

Item 1.
(a)	Name of issuer: Twist Bioscience Corp
(b)	Address of issuer's principal executive offices: 681 GATEWAY BLVD., 681 GATEWAY BLVD., SOUTH SAN FRANCISCO, CALIFORNIA, 94080
Item 2.
(a)	Name of person filing: William Blair Investment Management, LLC
(b)	Address or principal business office or, if none, residence: 150 North Riverside Plaza, Chicago, IL 60606
(c)	Citizenship: Delaware
(d)	Title of class of securities: Common Stock
(e)	CUSIP No.: 90184D100
Item 3.	If this statement is filed pursuant to §§ 240.13d-1(b) or 240.13d-2(b) or (c), check whether the person filing is a:
(a)	Broker or dealer registered under section 15 of the Act (15 U.S.C. 78o);
(b)	Bank as defined in section 3(a)(6) of the Act (15 U.S.C. 78c);
(c)	Insurance company as defined in section 3(a)(19) of the Act (15 U.S.C. 78c);
(d)	Investment company registered under section 8 of the Investment Company Act of 1940 (15 U.S.C. 80a-8);
(e)	An investment adviser in accordance with § 240.13d-1(b)(1)(ii)(E);
(f)	An employee benefit plan or endowment fund in accordance with § 240.13d-1(b)(1)(ii)(F);
(g)	A parent holding company or control person in accordance with § 240.13d-1(b)(1)(ii)(G);
(h)	A savings associations as defined in Section 3(b) of the Federal Deposit Insurance Act (12 U.S.C. 1813);
(i)	A church plan that is excluded from the definition of an investment company under section 3(c)(14) of the Investment Company Act of 1940 (15 U.S.C. 80a-3);
(j)	A non-U.S. institution in accordance with § 240.13d-1(b)(1)(ii)(J). If filing as a non-U.S. institution in accordance with § 240.13d-1(b)(1)(ii)(J), please specify the type of institution:
(k)	Group, in accordance with Rule 240.13d-1(b)(1)(ii)(K).
Item 4.	Ownership
(a)	Amount beneficially owned: 5,954,429
(b)	Percent of class: 9.7%
(c)	Number of shares as to which the person has:
	(i) Sole power to vote or to direct the vote: 5,459,833
	(ii) Shared power to vote or to direct the vote: 0
	(iii) Sole power to dispose or to direct the disposition of: 5,954,429
	(iv) Shared power to dispose or to direct the disposition of: 0
Item 5.	Ownership of 5 Percent or Less of a Class.
	Not Applicable
Item 6.	Ownership of more than 5 Percent on Behalf of Another Person.
	Not Applicable
Item 7.	Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on by the Parent Holding Company or Control Person.
	Not Applicable
Item 8.	Identification and Classification of Members of the Group.
	Not Applicable
Item 9.	Notice of Dissolution of Group.
	Not Applicable

Item 10.

Certifications:

By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under § 240.14a-11.

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

WILLIAM BLAIR INVESTMENT MANAGEMENT, LLC

Signature:	Telmo Martins
Name/Title:	Chief Compliance Officer
Date:	02/09/2026

FAQ

What ownership stake in Twist Bioscience (TWST) does William Blair Investment Management report?

William Blair Investment Management, LLC reports beneficial ownership of 5,954,429 Twist Bioscience common shares, representing 9.7% of the class. This stake is disclosed in Amendment No. 1 to a Schedule 13G with an event date of December 31, 2025.

How many Twist Bioscience (TWST) shares can William Blair vote and dispose of?

William Blair Investment Management, LLC reports sole voting power over 5,459,833 shares and sole dispositive power over 5,954,429 shares of Twist Bioscience common stock. It reports zero shared voting power and zero shared dispositive power over the securities.

What type of SEC filing did William Blair make regarding Twist Bioscience (TWST)?

William Blair Investment Management, LLC filed Amendment No. 1 to a Schedule 13G for Twist Bioscience Corp. This filing reports passive beneficial ownership of the company’s common stock as of December 31, 2025, rather than seeking to influence control.

On what date is William Blair’s reported Twist Bioscience (TWST) ownership effective?

The reported ownership is tied to a date of event of December 31, 2025. As of that date, William Blair Investment Management, LLC beneficially owned 5,954,429 Twist Bioscience common shares, representing 9.7% of the company’s outstanding common stock class.

Does William Blair state an intent to influence control of Twist Bioscience (TWST)?

The filer certifies the securities were acquired and are held in the ordinary course of business and not for the purpose or effect of changing or influencing control of Twist Bioscience, other than activities solely in connection with a nomination under Rule 14a-11.

Who signed the Twist Bioscience (TWST) Schedule 13G/A on behalf of William Blair?

The Schedule 13G/A for Twist Bioscience was signed by Telmo Martins, Chief Compliance Officer of William Blair Investment Management, LLC. The signature block certifies that the information in the statement is true, complete, and correct as of February 9, 2026.