Vanguard (TWST) amendment shows 0 shares after Jan 12, 2026 realignment

Rhea-AI Filing Summary

The Vanguard Group filed Amendment No. 5 to a Schedule 13G/A reporting 03/13/2026 activity for Twist Bioscience Corp common stock. The filing states that, after an internal realignment effective 01/12/2026, certain Vanguard subsidiaries will report ownership separately under SEC Release No. 34-39538. The filing shows amount beneficially owned: 0 and percent of class: 0%. The filing is signed by Ashley Grim, Head of Global Fund Administration, on 03/27/2026.

Insights

Institutional Ownership Analyst

Vanguard reports zero beneficial ownership in TWST after internal disaggregation.

The amendment documents an internal realignment dated 01/12/2026 and states subsidiaries will report separately in reliance on SEC Release No. 34-39538. The filing explicitly lists 0 shares beneficially owned and 0% of the class.

This indicates the reporting aggregation changed; subsequent Schedule 13G/A filings by Vanguard entities may show holdings. Follow future filings for any reappearance of Vanguard-affiliated holdings.

SEC Compliance Specialist

Amendment clarifies reliance on SEC Release No. 34-39538 for disaggregated reporting.

The document cites Release No. 34-39538 and explains that certain subsidiaries or business divisions will report separately. It affirms Vanguard no longer is deemed to beneficially own securities held by those subsidiaries after the realignment.

Signature block shows Ashley Grim signed on 03/27/2026. Future filings by the named subsidiaries should be checked for any reported positions.

SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

SCHEDULE 13G

UNDER THE SECURITIES EXCHANGE ACT OF 1934

(Amendment No. 5)

Twist Bioscience Corp

(Name of Issuer)

Common Stock

(Title of Class of Securities)

90184D100

(CUSIP Number)

03/13/2026

(Date of Event Which Requires Filing of this Statement)

Check the appropriate box to designate the rule pursuant to which this Schedule is filed:

Rule 13d-1(b)

Rule 13d-1(c)

Rule 13d-1(d)

schemaVersion:

SCHEDULE 13G

CUSIP Number(s): 90184D100

1

Names of Reporting Persons

The Vanguard Group

2

Check the appropriate box if a member of a Group (see instructions)

(a)
(b)

3

Sec Use Only

4

Citizenship or Place of Organization

PENNSYLVANIA

Number of Shares Beneficially Owned by Each Reporting Person With:

5	Sole Voting Power 0.00
6	Shared Voting Power 0.00
7	Sole Dispositive Power 0.00
8	Shared Dispositive Power 0.00

9

Aggregate Amount Beneficially Owned by Each Reporting Person

0.00

10

Check box if the aggregate amount in row (9) excludes certain shares (See Instructions)

11

Percent of class represented by amount in row (9)

0 %

12

Type of Reporting Person (See Instructions)

IA

Comment for Type of Reporting Person: On January 12, 2026, The Vanguard Group, Inc. went through an internal realignment. In accordance with SEC Release No. 34-39538 (January 12, 1998), certain subsidiaries or business divisions of subsidiaries of The Vanguard Group, Inc., that formerly had, or were deemed to have, beneficial ownership with The Vanguard Group, Inc., will report beneficial ownership separately (on a disaggregated basis) from The Vanguard Group, Inc. in reliance on such release. These subsidiaries and/or business divisions pursue the same investment strategies as previously pursued by The Vanguard Group, Inc. prior to the realignment. Further in accordance with SEC Release No. 34-39538 (January 12, 1998), The Vanguard Group, Inc. no longer has, or is deemed to have, beneficial ownership over securities beneficially owned by such subsidiaries and/or business divisions.

SCHEDULE 13G

Item 1.
(a)	Name of issuer: Twist Bioscience Corp
(b)	Address of issuer's principal executive offices: 681 Gateway Boulevard, San Francisco, CA, 94158
Item 2.
(a)	Name of person filing: The Vanguard Group
(b)	Address or principal business office or, if none, residence: 100 Vanguard Blvd.,, Malvern, PA, 19355
(c)	Citizenship: PA
(d)	Title of class of securities: Common Stock
(e)	CUSIP No.:
Item 3.	If this statement is filed pursuant to §§ 240.13d-1(b) or 240.13d-2(b) or (c), check whether the person filing is a:
(a)	Broker or dealer registered under section 15 of the Act (15 U.S.C. 78o);
(b)	Bank as defined in section 3(a)(6) of the Act (15 U.S.C. 78c);
(c)	Insurance company as defined in section 3(a)(19) of the Act (15 U.S.C. 78c);
(d)	Investment company registered under section 8 of the Investment Company Act of 1940 (15 U.S.C. 80a-8);
(e)	An investment adviser in accordance with § 240.13d-1(b)(1)(ii)(E);
(f)	An employee benefit plan or endowment fund in accordance with § 240.13d-1(b)(1)(ii)(F);
(g)	A parent holding company or control person in accordance with § 240.13d-1(b)(1)(ii)(G);
(h)	A savings associations as defined in Section 3(b) of the Federal Deposit Insurance Act (12 U.S.C. 1813);
(i)	A church plan that is excluded from the definition of an investment company under section 3(c)(14) of the Investment Company Act of 1940 (15 U.S.C. 80a-3);
(j)	A non-U.S. institution in accordance with § 240.13d-1(b)(1)(ii)(J). If filing as a non-U.S. institution in accordance with § 240.13d-1(b)(1)(ii)(J), please specify the type of institution:
(k)	Group, in accordance with Rule 240.13d-1(b)(1)(ii)(K).
Item 4.	Ownership
(a)	Amount beneficially owned: 0
(b)	Percent of class: 0 %
(c)	Number of shares as to which the person has:
	(i) Sole power to vote or to direct the vote: 0
	(ii) Shared power to vote or to direct the vote: 0
	(iii) Sole power to dispose or to direct the disposition of: 0
	(iv) Shared power to dispose or to direct the disposition of: 0
Item 5.	Ownership of 5 Percent or Less of a Class.
	Ownership of 5 percent or less of a class
Item 6.	Ownership of more than 5 Percent on Behalf of Another Person.
	If any other person is known to have the right to receive or the power to direct the receipt of dividends from, or the proceeds from the sale of, such securities, a statement to that effect should be included in response to this item and, if such interest relates to more than 5 percent of the class, such person should be identified. A listing of the shareholders of an investment company registered under the Investment Company Act of 1940 or the beneficiaries of employee benefit plan, pension fund or endowment fund is not required. The Vanguard Group, including investment companies registered under the Investment Company Act of 1940 and other managed accounts, have the right to receive or the power to direct the receipt of dividends from, or the proceeds from the sale of, the securities reported herein.No one other person's interest in the securities reported herein is more than 5%.
Item 7.	Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on by the Parent Holding Company or Control Person.
	Not Applicable
Item 8.	Identification and Classification of Members of the Group.
	Not Applicable
Item 9.	Notice of Dissolution of Group.
	Not Applicable

Item 10.

Certifications:

By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under § 240.14a-11.

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

The Vanguard Group

Signature:	Ashley Grim
Name/Title:	Head of Global Fund Administration
Date:	03/27/2026

FAQ

What does Vanguard's Amendment No. 5 say about its holdings in TWST?

The amendment states amount beneficially owned: 0 and percent of class: 0%. It reports an internal realignment and disaggregation under SEC Release No. 34-39538, with subsidiaries to report separately effective 01/12/2026.

Why is Vanguard reporting separate holdings after the January 12, 2026 realignment?

Vanguard explains it underwent an internal realignment on 01/12/2026 and, per SEC Release No. 34-39538, certain subsidiaries will report beneficial ownership separately rather than under The Vanguard Group, Inc.

Who signed the Schedule 13G/A amendment for Vanguard regarding TWST?

The amendment is signed by Ashley Grim, listed as Head of Global Fund Administration, with the signature date shown as 03/27/2026 on the filing cover information.

Does the filing indicate Vanguard still controls dividends or sale proceeds for TWST shares?

The filing states that Vanguard, including registered investment companies and managed accounts, has the right to receive or direct dividends or sale proceeds when applicable, but here it reports no beneficial ownership in TWST.

Will Vanguard or its subsidiaries file further reports for TWST?

The amendment indicates subsidiaries will report separately in reliance on Release No. 34-39538; subsequent Schedule 13G/A or similar filings by those subsidiaries may disclose holdings if any are acquired.