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Aethlon Medical Continues to Observe Directional Changes in Second Cohort of Australian Oncology Clinical Trial Evaluating Hemopurifier®

(Positive)

Aethlon Medical (Nasdaq: AEMD) reported that patients in the second cohort of its Australian oncology feasibility study of the investigational Hemopurifier showed biological changes similar to those seen in the first cohort after treatment. Replicating these biomarker shifts supports continued enrollment of the third and final cohort, after which an independent statistician will analyze combined data for potential dose-response patterns.

The study evaluates safety, feasibility and dosing of Hemopurifier in patients with advanced solid tumors progressing despite anti-PD-1 immunotherapies. Observed changes include reductions in several extracellular vesicle populations and tumor-associated microRNAs, improvements in multiple immune-related laboratory ratios, and increases in total and tumor-specific T-cell populations. Aethlon highlights that these are preliminary, descriptive observations from an early-stage feasibility study, not designed to demonstrate clinical efficacy, and that larger, adequately powered trials will be needed to assess clinical benefit.

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Positive

  • Consistent biomarker changes in two cohorts observed after Hemopurifier treatment in advanced solid tumor patients
  • Second cohort showed more consistent and persistent reductions in multiple extracellular vesicle types through eight-week follow-up
  • Immune-related ratios such as NLR, MLR, SII, MAR and LAI shifted in directions associated in literature with improved immunotherapy responses
  • T-cell populations including CD4, CD8 and CD137-positive tumor-specific T cells increased in all three second-cohort participants
  • Third cohort enrollment underway with the first participant completing three four-hour Hemopurifier sessions in one week
  • FDA Breakthrough Device Designation granted for advanced/metastatic cancer unresponsive to standard therapy and for certain life-threatening viral infections

Negative

  • Data remain preliminary, based only on descriptive observations from an early-stage feasibility study
  • No formal statistical analysis yet; independent statistician will assess only after all three cohorts are completed
  • Study not powered for efficacy, so no conclusions can be drawn about Hemopurifier’s impact on clinical outcomes
  • Small sample size to date, with biomarker findings limited to early cohorts in a feasibility setting
  • Future larger trials required with clinical efficacy endpoints before any potential clinical benefit can be established

News Explained

The third cohort is underway, but the release still provides no completed statistical analysis or clinical-efficacy result.

The July 13, 2026 release reports directional biomarker changes in the second cohort of Aethlon Medical’s Australian oncology feasibility study and says enrollment in the third and final cohort is underway. For the company’s program, this moves the study into its third cohort while leaving the planned independent statistical analysis pending.

The study is evaluating the Hemopurifier’s safety, feasibility, and dosing in patients with advanced solid tumors whose cancers progressed despite anti-PD-1 treatment. Although Aethlon says the repeated observations strengthen the scientific rationale, the release describes them as preliminary, descriptive observations from raw data; formal statistical analysis has not yet been performed, and the study was not designed to demonstrate clinical efficacy.

The stated next milestones are completion of all three cohorts, analysis of the combined data by an independent statistician, and later larger trials using clinical-efficacy endpoints.

Market reaction: AEMD -5.51% on Australian oncology feasibility trial update

-5.51%
2 alerts
-5.51% News Effect
+5.3% Peak Tracked
-6.7% Trough Tracked
-$109K Valuation Impact
$1.86M Market Cap
0.0x Rel. Volume

On the day this news was published, AEMD declined 5.51%, reflecting a notable negative market reaction. Argus tracked a peak move of +5.3% during that session. Argus tracked a trough of -6.7% from its starting point during tracking. Our momentum scanner triggered 2 alerts that day, indicating moderate trading interest and price volatility. This price movement removed approximately $109K from the company's valuation, bringing the market cap to $1.86M at that time.

Data tracked by StockTitan Argus on the day of publication.

Market Context

The stock moved -5.5% in the session following this news. A sharp decline could reflect skepticism d...
Analysis

The stock moved -5.5% in the session following this news. A sharp decline could reflect skepticism despite similar biomarker shifts now seen in two cohorts, especially given prior clinical-trial headlines averaged roughly -1.17% next-day moves. The study was not designed for efficacy, so any disappointment would be amplified by early-stage, non-registrational data and relatively low short interest constraining forced buying.

Key Figures

Study cohorts: 3 patient cohorts Follow-up duration: 8-week follow-up MicroRNAs reduced: 2 microRNAs +4 more
7 metrics
Study cohorts 3 patient cohorts Australian oncology feasibility study design
Follow-up duration 8-week follow-up Persistence of biomarker changes in second cohort
MicroRNAs reduced 2 microRNAs Biomarkers associated with tumor growth and invasion
Participants in cohort 3 participants Immune cell changes observed in second cohort
Hemopurifier treatments 3 treatments First participant in third cohort over one week
Treatment duration 4-hour treatments Hemopurifier sessions in third cohort
Treatment period 1-week period Timing for three Hemopurifier treatments in third cohort

Previous Clinical trial Reports

5 past events · Latest: Mar 24 (Positive)
Same Type Pattern 5 events
Date Event Sentiment 24h Move Catalyst
Mar 24 DSMB safety review Positive -6.1% DSMB found no safety concerns and recommended advancing to final cohort.
Oct 08 Early cohort data Neutral +4.0% Early observations from first three oncology patients showed directional EV and microRNA changes.
Oct 07 Cohort 1 biomarker data Neutral -5.8% Preliminary Hemopurifier biomarker shifts reported in first cohort with extensive caveats.
Jul 15 DSMB review cohort 1 Positive +4.3% DSMB saw no safety issues in initial cohort and recommended advancing to cohort 2.
May 14 Preclinical EV removal data Positive -2.3% Preclinical data showed strong removal of platelet-derived EVs supporting oncology trial rationale.

24h Move is the share-price change in the day after each event; other market factors may also have contributed.

Pattern Detected

For prior clinical-trial updates, Aethlon’s share-price reaction has been mixed, with several positive safety or biomarker updates followed by flat-to-negative next-day moves on average.

Key Terms

extracellular vesicles, micrornas, neutrophil-to-lymphocyte ratio, systemic immune-inflammation index, +1 more
5 terms
extracellular vesicles medical
"Reductions in tumor-derived extracellular vesicles (EVs), platelet-derived EVs"
Extracellular vesicles are tiny, cell-made particles—think microscopic bubbles—that carry proteins, genetic material and signals between cells. Investors care because these vesicles can be used as disease markers, drug-delivery vehicles, or therapeutic products; that creates opportunities and risks around clinical validation, manufacturing scale-up, intellectual property and regulatory approval that can materially affect the value of biotech and diagnostics companies.
micrornas medical
"Reductions in two microRNAs that have been associated with tumor growth"
microRNAs are tiny pieces of genetic material that act like dimmer switches for genes, turning down the production of specific proteins inside cells. Investors pay attention because microRNAs can be used as diagnostic markers or drug targets—meaning discoveries can create new tests, treatments, or business opportunities, and progress or setbacks in that research can materially affect a company’s clinical prospects and valuation.
neutrophil-to-lymphocyte ratio medical
"including neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio"
The neutrophil-to-lymphocyte ratio (NLR) is a simple number calculated from a routine blood test that compares two types of white blood cells: neutrophils (first responders in inflammation) and lymphocytes (cells tied to long-term immune response). Like measuring the relative sizes of two teams in a tug-of-war, a higher NLR often signals more systemic inflammation or stress and can predict disease severity or treatment response — information investors use to gauge the likely success, market potential, or regulatory risk of medical therapies and clinical trials.
systemic immune-inflammation index medical
"monocyte-to-lymphocyte ratio (MLR), Systemic Immune-Inflammation Index (SII)"
A blood-based score calculated from routine counts of neutrophils, lymphocytes, and platelets that summarizes a person’s overall immune response and level of inflammation. Think of it as a single dashboard light that combines several gauges to indicate whether the body is in an inflammatory or immune-activated state. It matters to investors because it is often used as a marker in clinical studies, diagnostic tests, and drug development to predict disease severity, treatment response, or patient outcomes.
pd-l1 medical
"Reductions in tumor-derived extracellular vesicles (EVs)... and PD-L1-positive EVs"
PD-L1 is a protein found on the surface of some cells that acts like a stop sign for the immune system, telling certain immune cells to back off. It matters to investors because many cancer drugs and diagnostic tests target or measure PD-L1 to unlock immune responses or predict which patients will benefit, affecting clinical success, regulatory approval, and potential sales in the oncology market.

AI-generated analysis. How Rhea-AI works. Not financial advice.

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Second cohort findings consistent with previously reported observations; independent statistical analysis planned following completion of all three patient cohorts

SAN DIEGO, July 13, 2026 /PRNewswire/ -- Aethlon Medical, Inc. (Nasdaq: AEMD), a medical therapeutic company developing products to treat cancer and life-threatening viral infections, today announced that patients in the second cohort of its Australian oncology feasibility study demonstrated biological changes consistent with those previously observed in the first cohort following treatment with the investigational Hemopurifier®. Replicating these findings in a second group of patients strengthens the scientific rationale for the Company's ongoing clinical program and supports continued enrollment in the third and final cohort before an independent statistical analysis is conducted.

"We are encouraged to observe similar directional changes across multiple biomarkers in both the first and second patient cohorts," said James Frakes, Chief Executive Officer and Chief Financial Officer of Aethlon Medical. " Observing these directional changes in a second patient cohort builds upon our initial observations and provides additional data as we advance toward completion of the third cohort. While these are preliminary findings from an early feasibility study, they represent an important milestone as we advance toward completion of the third cohort and an independent statistical analysis to determine whether these observations represent a dose-response to Hemopurifier treatment."

The Australian feasibility study is evaluating the safety, feasibility and dosing of the Hemopurifier® in patients with advanced solid tumors whose cancers have progressed despite treatment with anti-PD-1 immunotherapies.

The second cohort continued to show directional changes in several biomarkers that researchers believe are associated with tumor growth, immune suppression and response to immunotherapy, including:

  • Reductions in tumor-derived extracellular vesicles (EVs), platelet-derived EVs and PD-L1-positive EVs, with these changes appearing more consistently across all participants than in the first cohort and generally persisting through the eight-week follow-up period.
  • Reductions in two microRNAs that have been associated with tumor growth and cancer invasion.
  • Improvements in multiple immune-related laboratory ratios—including neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), Systemic Immune-Inflammation Index (SII), monocyte-to-albumin ratio (MAR) and Lymphocyte Albumin Index (LAI)—that have been associated in published research with improved responses to immunotherapy.
  • Increases in total T cells, CD4 and CD8 T-cell populations, and tumor-specific CD137-positive T cells in all three participants, with these changes generally persisting through the eight-week follow-up period.

Taken together, the first two cohorts have now shown similar directional changes across multiple biological markers following Hemopurifier treatment. Enrollment in the third cohort is underway, with the first participant having already completed three four-hour Hemopurifier treatments during a one-week period. After all three cohorts are complete, an independent statistician will analyze the combined data to determine whether these observations support a dose-response relationship.

EVs continue to be recognized as important drivers of cancer progression and resistance to checkpoint inhibitors such as Keytruda® and Opdivo®. The Hemopurifier® is designed to remove these tumor-derived vesicles from the bloodstream, with the goal of reducing immune suppression and potentially enhancing the body's ability to fight cancer.

"We believe the Hemopurifier has the ability to complement existing therapies rather than replace them and could be a platform technology with potential applications across multiple disease areas such as oncology, infectious diseases, and future emerging threats. We believe there exists a 'pipeline within a single device,'" said Mr. Frakes.

Advancing a Novel Approach to Cancer Immunotherapy

Extracellular vesicles released by tumors continue to be recognized as important drivers of cancer progression, metastasis and resistance to immunotherapy. EVs expressing PD-L1 have been associated with resistance to checkpoint inhibitors, including Keytruda® and Opdivo®. The Hemopurifier is designed to remove tumor-derived EVs and other pathogenic particles from circulation, potentially reducing immunosuppressive signaling and improving the body's anti-tumor immune response.

The Hemopurifier has received FDA Breakthrough Device Designation for the treatment of patients with advanced or metastatic cancer who are unresponsive or intolerant to standard-of-care therapy, as well as for the treatment of life-threatening viral infections not addressed by approved therapies.

Important Study Limitations

The Company cautions that these findings represent descriptive observations from raw data generated in an early-stage feasibility study. Formal statistical analyses have not yet been performed and will be conducted only after completion of all three study cohorts by an independent statistician.

The study was not designed to demonstrate clinical efficacy, and no conclusions can be drawn regarding the Hemopurifier's impact on patient outcomes. Larger, adequately powered clinical trials designed with clinical efficacy endpoints will be required to determine whether the biological changes observed translate into meaningful clinical benefit.

About Aethlon Medical

Aethlon Medical, Inc. is developing the Hemopurifier®, an investigational extracorporeal therapeutic designed to remove tumor-derived extracellular vesicles and enveloped viruses from the bloodstream. The Company is advancing the Hemopurifier in oncology and life-threatening viral infections with the goal of improving outcomes for patients with limited treatment options.

Forward-Looking Statements

This press release contains forward-looking statements within the meaning of Section 27A of the Securities Act of 1933 and Section 21E of the Securities Exchange Act of 1934 that involve risks and uncertainties. Statements containing words such as "may," "believe," "anticipate," "expect," "intend," "plan," "project," "will," "projections," "estimate," "potentially," or similar expressions constitute forward-looking statements. Such forward looking statements include but are not limited to statements regarding the completion and timing of enrollment in the third cohort of the Australian oncology feasibility study; the timing and results of the independent statistical analysis; whether the biological changes observed following Hemopurifier® treatment are reproducible or support a dose-response relationship; the potential safety, feasibility or biological activity of the Hemopurifier; the ability of the Hemopurifier to remove tumor-derived extracellular vesicles or otherwise modulate immune function; whether the biological changes observed will translate into meaningful clinical benefit or improved patient outcomes; the potential for the Hemopurifier to complement existing cancer therapies or improve responses to immunotherapy; the future development, regulatory progress, clinical evaluation, commercialization or market acceptance of the Hemopurifier; the potential applicability of the Hemopurifier in oncology, viral infections or other disease indications; and the Company's plans, expectations and objectives for future clinical studies and development activities.. Such forward-looking statements are subject to significant risks and uncertainties, and actual results may differ materially from the results anticipated in the forward-looking statements. These forward-looking statements are based upon Aethlon's current expectations and involve assumptions that may never materialize or may prove to be incorrect. The foregoing list of risks and uncertainties is illustrative, but is not exhaustive. Additional factors that could cause results to differ materially from those anticipated in forward-looking statements can be found under the caption "Risk Factors" in the Company's Annual Report on Form 10-K for the year ended March 31, 2026, and in the Company's other filings with the Securities and Exchange Commission, including its quarterly Reports on Form 10-Q. All forward-looking statements contained in this press release speak only as of the date on which they were made. Except as may be required by law, the Company does not intend, nor does it undertake any duty, to update this information to reflect future events or circumstances. The clinical findings described herein are preliminary in nature, have not been subjected to formal statistical analysis, and may not be be replicated in subsequent clinical studies or trials.

Company Contact:
Jim Frakes
Chief Executive Officer and Chief Financial Officer
Aethlon Medical, Inc.
Jfrakes@aethlonmedical.com

Investor Contact:
Susan Noonan
S.A. Noonan Communications, LLC
susan@sanoonan.com

Cision View original content:https://www.prnewswire.com/news-releases/aethlon-medical-continues-to-observe-directional-changes-in-second-cohort-of-australian-oncology-clinical-trial-evaluating-hemopurifier-302823192.html

SOURCE Aethlon Medical, Inc.

FAQ

What did Aethlon Medical (AEMD) report from the second cohort of its Australian Hemopurifier cancer trial?

Aethlon Medical reported that the second cohort showed biomarker changes similar to the first cohort after Hemopurifier treatment. According to Aethlon Medical, these directional shifts across multiple biomarkers support continued enrollment of the third cohort before an independent statistical analysis of combined data.

What biomarkers changed in Aethlon Medical’s Australian Hemopurifier oncology feasibility study (AEMD)?

The study observed reductions in several extracellular vesicle types and two tumor-associated microRNAs, plus improvements in multiple immune-related ratios. According to Aethlon Medical, increases in total T cells, CD4, CD8 and tumor-specific CD137-positive T cells also persisted through an eight-week follow-up period.

Does Aethlon Medical’s Hemopurifier show proven clinical efficacy in the Australian oncology trial?

No, the current feasibility study was not designed to demonstrate clinical efficacy, so no outcome conclusions are possible. According to Aethlon Medical, the findings are descriptive biomarker observations, and larger, adequately powered trials with clinical endpoints will be required to assess potential patient benefit.

What is the design and goal of Aethlon Medical’s Australian Hemopurifier study in advanced solid tumors (AEMD)?

The Australian feasibility study evaluates safety, feasibility and dosing of Hemopurifier in advanced solid tumor patients progressing after anti-PD-1 therapy. According to Aethlon Medical, three cohorts receive Hemopurifier treatments, with an independent statistician analyzing combined cohort data after study completion.

How does Aethlon Medical’s Hemopurifier aim to help cancer patients treated with immunotherapy?

Hemopurifier is designed to remove tumor-derived extracellular vesicles and other pathogenic particles from circulation. According to Aethlon Medical, this may reduce immunosuppressive signaling and could potentially complement checkpoint inhibitors like Keytruda and Opdivo by supporting the body’s anti-tumor immune response.

What regulatory designations has the Hemopurifier received that are relevant for Aethlon Medical (AEMD) investors?

Hemopurifier has received FDA Breakthrough Device Designation for advanced or metastatic cancer unresponsive or intolerant to standard-of-care therapy. According to Aethlon Medical, it also holds this designation for life-threatening viral infections not addressed by approved therapies, highlighting its potential multi-indication platform.

What are the key limitations of the current Hemopurifier oncology data reported by Aethlon Medical?

The data come from an early-stage feasibility study using descriptive, raw biomarker observations without formal statistics. According to Aethlon Medical, the trial was not designed for efficacy, and only future, larger studies with clinical endpoints can determine whether these biological changes translate into clinical benefit.