Tharimmune Announces Positive Target Engagement with Novel Biparatopic PD-1/VEGF and Multispecific HER2/HER3 Biologics Leveraging Proprietary EpiClick Technology

Rhea-AI Summary

Tharimmune (NASDAQ:THAR) has expanded its product pipeline with HS1940, a novel biparatopic biologic targeting both PD-1 and VEGF receptors using their proprietary EpiClick Technology. HS1940 is designed to simultaneously engage the PD-1 pathway and inhibit angiogenesis, potentially offering improved treatment options for multiple cancer types.

The company has generated positive target engagement data showing that the biparatopic format provides enhanced therapeutic versatility compared to monospecific antibodies. Tharimmune plans to initiate IND-enabling studies for HS1940 throughout 2025.

Additionally, Tharimmune is developing HS3215, a multispecific antibody targeting HER2 and HER3 cancer drivers. This approach leverages unique epitopes not addressed by existing drugs, potentially overcoming resistance mechanisms associated with current HER2-targeted therapies.

Positive

• Positive target engagement data achieved for HS1940
• Novel drug candidate targeting previously undruggable PD-1 epitopes
• Dual-action mechanism potentially offering improved cancer treatment efficacy
• Pipeline expansion with two promising candidates (HS1940 and HS3215)

Negative

• Products still in early development phase
• IND-enabling studies not yet initiated
• No clinical trial data available

BRIDGEWATER, NJ / ACCESS Newswire / March 4, 2025 / Tharimmune, Inc. (NASDAQ:THAR) ("Tharimmune" or the "Company"), a clinical-stage biotechnology company focused on immunology and inflammation, today announced the expansion of its product pipeline with HS1940, a dual-target multispecific biologic engineered to bind to both Programmed Death-1 (PD-1) and Vascular Endothelial Growth Factor (VEGF) receptors. Using its proprietary EpiClick^™ Technology, a versatile multispecific antibody engineering platform, HS1940 represents a key expansion of Tharimmune's product pipeline and underscores the Company's commitment to addressing unmet needs.

HS1940 is designed as a biparatopic (binding two epitopes on a single target) biologic, simultaneously engaging the PD-1 pathway and inhibiting angiogenesis. By using multiple, previously undruggable epitopes on PD-1, and blocking VEGF-mediated tumor vascularization, HS1940 may broaden treatment options and improve outcomes across multiple types of cancer and may access receptor regions that other PD-1 inhibitors (e.g., nivolumab and pembrolizumab) may not reach.

"We are extremely excited about the potential and versatility of our EpiClick platform. The implications for therapeutic intervention are potentially substantial, suggesting EpiClick-derived biologics may offer more potent and nuanced approaches to disease modulation," said Randy Milby, CEO of Tharimmune. "With EpiClick, we can strategically combine antibody binding domains to target multiple hard-to-reach epitopes, creating highly customized therapeutics tailored to the specifics of tumor destruction. For example, by addressing novel and multiple PD-1 epitopes while simultaneously inhibiting VEGF, we have the potential to offer a differentiated and more effective treatment option for a variety of solid tumors."

EpiClick enables the rapid and efficient creation of modular antibodies capable of high specificity and affinity toward multiple targets. A key feature of EpiClick is its "mix and match" approach, allowing distinct antibody binding domains - including those derived from previously inaccessible, undruggable epitopes - to be combined in either small-format or full-length configurations.

In the HS1940 family, the anti-PD-1 components leverage a novel design inspired by bovine antibodies, enabling them to target previously undruggable PD-1 epitopes. The anti-VEGF component, and the overall multispecific format, are engineered using EpiClick's modular design capabilities to create novel biparatopic PD-1 binding domains engineered on multiple VEGF scaffolds, as illustrated below.

The Company has generated positive target engagement data showing that the biparatopic format expands therapeutic versatility compared with monospecific antibodies, which are limited to targeting a single epitope on a single receptor. As illustrated, the biparatopic antibody constructs effectively achieve target engagement on both PD-1 and VEGF. By engaging two distinct cellular pathways, HS1940 has the potential to elicit complex and synergistic anti-tumor effects. Tharimmune plans to continue preclinical testing with HS1940 and present data at future scientific conferences. The Company expects to initiate IND-enabling studies for HS1940 throughout 2025.

PD-1 is a well-validated immune checkpoint receptor that, when activated, suppresses T-cell function and allows cancer cells to evade immune detection. VEGF drives angiogenesis, which provides a nutrient and oxygen supply for tumors. By simultaneously blocking both pathways, HS1940 aims to achieve a synergistic anti-tumor effect by blocking PD-1, which releases immune "brakes," and enhancing T-cell-mediated tumor attack. Blocking VEGF disrupts tumor vasculature, starving them of nutrients.

Building upon the EpiClick platform, Tharimmune is also developing a new generation of multispecific antibodies targeting HER2 and HER3, two validated drivers of cancer growth and metastases. While HER2 is the focus of numerous successful commercial therapies, Tharimmune's approach offers distinct advantages. EpiClick leverages the "knob-and-stalk" from bovine-derived antibodies engineered to reach unique HER2 epitopes not addressed by existing drugs, while simultaneously engaging HER3. This dual engagement has the potential to disrupt cancer signaling in novel ways and overcome resistance to mechanisms associated with existing HER2-targeted therapies. By targeting distinct epitopes and incorporating HER3 engagement, Tharimmune's EpiClick-derived antibodies such as HS3215 offer a promising new avenue for more effective and targeted cancer treatments. Tharimmune is conducting preclinical studies to evaluate and optimize HS3215, with plans to advance the molecule into clinical trials following IND-enabling studies.

About EpiClick^™ Technology
EpiClick^™ Technology is a platform for creating customizable, multispecific antibodies that target previously undruggable epitopes, including those on PD-1, HER2, HER3 and other validated cancer targets. Inspired by bovine antibodies' unique "knob-and-stalk" structure, EpiClick uses engineered "knob" domains - small, precise binding units - that can "click" into recessed protein sites inaccessible to conventional antibodies. Its modular nature allows these "knobs," each targeting a specific epitope, to be paired with additional antibody components, creating a vast combinatorial library of multispecific therapeutics. For example, EpiClick enables the creation of novel biparatopic anti-PD-1 components, as used in HS1940, or HER2/HER3 domains, as in HS3215. By unlocking undruggable epitopes, EpiClick aims to deliver more effective and targeted treatments in immunotherapy and cancer therapy.

About Tharimmune, Inc.
Tharimmune is a clinical-stage biotechnology company developing a diverse portfolio of therapeutic candidates in immunology, inflammation and oncology. Its lead clinical asset, TH104, aims to suppress chronic pruritus associated with primary biliary cholangitis (PBC), a rare autoimmune liver disease with no known cure. The expanded pipeline includes TH023, an oral TNF-alpha inhibitor offering a new approach to treating autoimmune diseases. Tharimmune is also advancing early-stage multispecific biologics targeting unique epitopes against multiple solid tumors through its proprietary EpiClick^™ Technology. The company has a license agreement with OmniAb, Inc. to access their antibody discovery technology for targeting specified disease markers. For more information, please visit: www.tharimmune.com.

Forward-Looking Statements
Certain statements in this press release are forward-looking within the meaning of the Private Securities Litigation Reform Act of 1995. All statements, other than statements of historical facts, contained in this press release, including statements regarding the timing and design of Tharimmune's future Phase 2 trial, Tharimmune's strategy, future operations, future financial position, projected costs, prospects, plans and objectives of management, are forward-looking statements. The words "anticipate," "believe," "continue," "could," "depends," "estimate," "expect," "intend," "may," "ongoing," "plan," "potential," "predict," "project," "target," "should," "will," "would," and similar expressions are intended to identify forward-looking statements, although not all forward-looking statements contain these identifying words. The Company may not actually achieve the plans, intentions, or expectations disclosed in these forward-looking statements, and you should not place undue reliance on these forward-looking statements. Actual results or events could differ materially from the plans, intentions and expectations disclosed in these forward-looking statements. Factors that may cause such differences, include, but are not limited to, those discussed under Risk Factors set forth in our Annual Report on Form 10-K for the year ended December 31, 2023 and other periodic reports filed by the Company from time to time with the Securities and Exchange Commission. In addition, the forward-looking statements included in this press release represent the Company's views as of the date of this release. Subsequent events and developments may cause the Company's views to change; however, the Company does not undertake and specifically disclaims any obligation to update or revise any forward-looking statements to reflect new information, future events or circumstances or to reflect the occurrences of unanticipated events, except as may be required by applicable law. These forward-looking statements should not be relied upon as representing the Company's views as of any date subsequent to the date of this release.

Contacts
Tharimmune, Inc.
ir@tharimmune.com

Alliance Advisors IR
Tirth T. Patel
tpatel@allianceadvisors.com
212-201-6614

SOURCE: Tharimmune Inc.

View the original press release on ACCESS Newswire

FAQ

What is Tharimmune's new HS1940 drug candidate and how does it work?

HS1940 is a dual-target biologic that binds to both PD-1 and VEGF receptors, blocking immune checkpoints and tumor blood vessel formation simultaneously. It uses novel epitopes unreachable by current PD-1 inhibitors.

When will Tharimmune (THAR) begin IND-enabling studies for HS1940?

Tharimmune plans to initiate IND-enabling studies for HS1940 throughout 2025.

How does Tharimmune's EpiClick Technology platform work in drug development?

EpiClick enables rapid creation of modular antibodies with high specificity for multiple targets, using a 'mix and match' approach to combine different antibody binding domains in various configurations.

What advantages does Tharimmune's HS3215 offer over existing HER2 cancer therapies?

HS3215 targets unique HER2 epitopes unreached by existing drugs while simultaneously engaging HER3, potentially overcoming resistance to current HER2-targeted therapies.