AMENDMENT NO. 2 DATED OCTOBER 21, 2026
To Prospectus Supplement dated July 17, 2026
(To Prospectus Dated June 15, 2026) |
Filed pursuant to Rule 424(b)(5)
Registration No. 333-296550 |

NANOVIRICIDES, INC.
Up to $2,374,774
Common Stock
This Amendment No. 2 to Prospectus
Supplement (this “Amendment”) amends and supplements the information in our prospectus, dated June 15, 2026 (the “Prospectus”),
and the prospectus supplement, dated July 17, 2026 and amended on July 27, 2026 (the “Prospectus Supplement”), which was filed
pursuant to our registration statement on Form S-3 (File No. 333-296550) (the “Registration Statement”). This Amendment should
be read in conjunction with the Prospectus and Prospectus Supplement, and is qualified by reference thereto, except to the extent that
the information herein amends or supersedes the information contained in the Prospectus and the Prospectus Supplement. This Amendment
is not complete without, and may only be delivered or utilized in connection with, the Prospectus and Prospectus Supplement, and any future
amendments or supplements thereto.
This Amendment is being filed
in connection with our previously announced “at the market offering” program, and to update the maximum amount of shares of
our common stock, par value $0.00001 per share, that we may issue and sell from time to time through or to D. Boral Capital LLC (the “Agent”)
as sales agent or principal, pursuant to the terms of our previously announced At Market Issuance Sales Agreement, dated July 17, 2026,
between us and the Agent (the “Sales Agreement”).
Our common stock is listed
on the New York Stock Exchange, or NYSE, under the symbol “NNVC”. As of October 19, 2026, the aggregate market value of our
common stock held by non-affiliates, or the public float, pursuant to General Instruction I.B.6 of Form S-3 was $43,161,651, which was
calculated based on 25,539,439 shares of our common stock outstanding held by non-affiliates as of October 19, 2026 at a price of $1.69
per share, the closing sale price for our common stock on September 21, 2026. As of the date hereof, we have offered and sold approximately
$12,012,443 of shares of our common stock pursuant to General Instruction I.B.6 of Form S-3 during the prior 12 calendar month period
that ends on and includes the date hereof. Pursuant to General Instruction I.B.6 of Form S-3, in no event will we sell securities in public
primary offerings on Form S-3 with a value exceeding one-third of our public float (as defined by General Instruction I.B.6) in any 12-calendar
month period so long as our public float remains below $75 million.
We are filing this Amendment to amend the Prospectus Supplement to
update the maximum amount we are eligible to sell under our Registration Statement pursuant to General Instruction I.B.6 of Form S-3.
Accordingly, in accordance with the terms of the Sales Agreement, we may currently offer and sell common stock through or to the Agent
having an aggregate offering price of up to $2,374,774 pursuant to the Prospectus and Prospectus Supplement, as amended by this Amendment.
In the event that our public float increases such that we may sell additional amounts under the Sales Agreement, Prospectus and Prospectus
Supplement, we will file another amendment to the Prospectus Supplement prior to making additional sales.
Investing in our common
stock involves risks. You should carefully consider the risks described under “Risk Factors” in the Prospectus and Prospectus
Supplement, as well as those described in our other reports and documents we filed with the Securities and Exchange Commission (the “SEC”)
that we incorporate by reference in the Prospectus, the Prospectus Supplement and this Amendment, before making a decision to invest in
our common stock.
Neither the SEC, any state
securities commission, nor any other regulatory body has approved or disapproved of these securities or determined if this Amendment is
accurate or complete. Any representation to the contrary is a criminal offense.
The date of this Amendment No. 2 to Prospectus
Supplement is October 21, 2026.